This article presents the results of the laboratory test program for stainless steel I-beams with sinusoidal corrugated web. Due to the web corrugation, the out-of-plane stiffness of the web increases compared to traditional flat web beams, so the thickness of the web can be reduced. Using a sinusoidal corrugated web in stainless steel beams is unique worldwide. Consequently, it is necessary to examine whether the currently available design method from the EN 1993-1-5:2006 is applicable. Three-point bending tests were conduct-ed to determine the shear resistance on beams with different geometrical parameters. The advanced numerical models were validated with the results of the laboratory tests. A parametrical study was conducted on beams with different geometrical parameters in order to investigate the shear resistance of the beams. The result of the parametrical research shows that the availa-ble design recommendation can also be used to determine the shear re-sistance of stainless steel I-beams with sinusoidal web corrugation.